Product Data Sheet

Standard heat-shock transformation of chem ically compe tent bacteria

1. Take competent cells out of -80°C and thaw on ice.
2. Take agar plates (containing the appropriate antibiotic) out of 4°C to warm up to room temperature or place in 37°C incubator.
3. Mix 5μl of DNA into 100μL competent cells in a microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few times.
4. Place the competent cell/DNA mixture on ice for 20-30min.
5. Heat shock each transformation tube by placing the bottom 2/3 of the tube into a 42°C water bath for 42 seconds. Put the tubes back on ice for 2 min.
6. Add 250-500μl SOC media (without antibiotic) and grow in 37°C shaking incubator for 45min.
7. Plate all of the transformation onto a 10cm LB agar plate containing the appropriate antibiotic.
8. Incubate plates at 37°C overnight.

载体序列：

LOCUS       AF053461                5688 bp    DNA     circular SYN 26-JAN-1999

DEFINITION  Reporter vector pCRE-Luc, complete sequence.

ACCESSION   AF053461

VERSION     AF053461.1  GI:4186171

KEYWORDS    .

SOURCE      Reporter vector pCRE-Luc

  ORGANISM  Reporter vector pCRE-Luc

            other sequences; artificial sequences; vectors.

REFERENCE   1  (bases 1 to 5688)

  AUTHORS   Zheng,C.-F.

  TITLE     pCRE-Luc plasmid

  JOURNAL   Unpublished

REFERENCE   2  (bases 1 to 5688)

  AUTHORS   Zheng,C.-F.

  TITLE     Direct Submission

  JOURNAL   Submitted (12-MAR-1998) Technical Services, Stratagene, 11011 N.

            Torrey Pines, San Diego, CA 92037, USA

REFERENCE   3  (bases 1 to 5688)

  AUTHORS   Zheng,C.-F.

  TITLE     Direct Submission

  JOURNAL   Submitted (29-OCT-1998) Technical Services, Stratagene, 11011 N.

            Torrey Pines, San Diego, CA 92037, USA

  REMARK    Sequence update by submitter

REFERENCE   4  (bases 1 to 5688)

  AUTHORS   Zheng,C.-F.

  TITLE     Direct Submission

  JOURNAL   Submitted (26-JAN-1999) Technical Services, Stratagene, 11011 N.

            Torrey Pines, San Diego, CA 92037, USA

  REMARK    Sequence update by submitter